Simultaneous determination of urinary homovanillic acid, vanillylmandelic acid and creatinine by HPLC with column switching technique.
نویسندگان
چکیده
منابع مشابه
Creatinine related reference ranges for urinary homovanillic acid and vanillylmandelic acid at 6 months of age.
The relationship between homovanillic acid (HVA), vanillylmandelic acid (VMA), and creatinine in the urine of 6 month old babies has been studied and reference ranges in the form of centiles constructed for HVA and VMA against creatinine. Over 10,000 urine samples were collected from babies in four health districts in the north of England. HVA and VMA concentration, either independently or when...
متن کاملSeparation and determination of homovanillic acid and vanillylmandelic acid by capillary electrophoresis with electrochemical detection
A method of separation and determination of homovanillic acid (HVA) and vanillylmandelic acid (VMA) was developed based on capillary zone electrophoresis/amperometric detection with high sensitivity, good resolution and selectivity. In order to achieve complete separation and good response, several factors including pH, buffer concentration, separation voltage, detection potential and the lengt...
متن کاملLiquid-chromatographic assay of urinary vanillylmandelic acid and homovanillic acid, with clean-up by on-column injection of acetonitrile or methanol.
To separately measure vanillylmandelic acid (VMA) and homovanillic acid (HVA) in urine, the sample is diluted 10-fold with 50 mmol/L phosphoric acid, then exactly 5 microL is injected directly onto a 50 X 4.6 mm column of Nucleosil 3C18. Samples can be injected at 5-min intervals because all peaks after these compounds of interest are washed away. VMA is eluted from the column after 2.8 min wit...
متن کاملDetermination of urinary vanilmandelic acid and homovanillic acid by high performance liquid chromatography with amperometric detection.
A method was developed for the simultaneous determination of urinary vanilmandelic acid and homovanillic acid, which included a two step prepurification and a reversed-phase high-performance liquid chromatography with amperometric detection. Conditions were evaluated for performing measurements with the amperometric detector free from electric interference. The method was linear between 2.5 and...
متن کاملDetermination of urinary 3-methoxy-4-hydroxymandelic acid (vanillylmandelic acid) by paper electrophoresis.
called vanillylmandelic acid (VMA), in urine has recently come into use as a confirmatory test for pheochromocytoma (1-6). Armstrong and his associates (1, 2, 7) discovered that this phenolic acid occurred in small amounts in normal human urine and in elevated concentrations in pheochromocytoma. As a result of their observations (1, 2) and especially of those by others in related investigations...
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ژورنال
عنوان ژورنال: BUNSEKI KAGAKU
سال: 1990
ISSN: 0525-1931
DOI: 10.2116/bunsekikagaku.39.6_347